TGFA Antibody orb1252696 from Biorbyt

Product Specs
ItemTGFA Antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb1252696
ClonalityMonoclonal
ReactivityBovine, Human, Mouse, Rat
FormatLiquid
ApplicationsFC, IF, IHC, WB
ImmunogenA 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody
TargetTGFA
IsotypeIgG1, kappa
Conjugate/TagUnconjugated
HostMouse
Quantity100 µg
Concentration0.2 mg/mL
NCBI Full Gene Nametransforming growth factor alpha
NCBI Gene AliasesTFGA
CloneMF9
StorageAliquot and Store at 2-8°C. Avoid freez-thaw cycles

Specifications/Features

Application Notes: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

Application Dilutions: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

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